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Selection and Characterization of Interferon Response Mutants from Mouse L929 Fibroblast Cells

Anne L. Z. Sakuragi

Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, U.S.A.

Interferon (IFN) response mutants were selected from mouse L929 fibroblast cells and their specific resistance to is-1, an IFN-sensitive mutant of mengovirus, was studied. The standard L cell subline used in our laboratory (G3), is resistant to is-1 infection after pretreatment with low levels of IFN. Two clonal sublines that support the growth of is-1 in the presence of IFN (AS-4 and TA-6) were isolated from it, and two revertant lines (AS-4R1 and TA-6R1) were subsequently selected from AS-4 and TA-6. The kinetics of is-1 growth in the presence of IFN were found to vary in each of these sublines. Specific resistance to is-1 cannot be accounted for by enhanced induction of IFN, ability to bind IFN, or increased 2'-5'-oligo(A)-dependent endonuclease activity. AS-4 and TA-6 appear to have arisen through loss of one or more whole chromosomes. The origin of TA-6R1 is unclear.

Keywords: IFN-responsive, mouse L cells, mengovirus

Present address: Department of Biochemistry, University of Washington, Seattle, Washington 98195, U.S.A.

Received 6 August 1987; accepted 19 October 1987.