| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Molecular Virology, James N. Gamble Institute of Medical Research, Cincinnati, Ohio 45219, U.S.A.
The kinetics of synthesis of [35S]methionine-labelled respiratory syncytial virus-specific proteins were studied in CV-1 cells infected at high multiplicity. Immunoprecipitated viral proteins resolved by SDS-PAGE were quantified by scanning fluorographs of protein bands. The nucleocapsid (N) protein was detectable by 2 h post-infection (p.i.), whereas the phospho- (P), matrix (M) and fusion (F0) proteins and Vp24 (a matrix-like protein) were first detected between 4 and 6 h p.i. Synthesis of the glyco- (G) protein was first detected at 6 h p.i. and reached its peak synthesis rate at 10 h p.i. Virus-specific P, M and Vp24 proteins were phosphorylated in infected cells. The P protein was highly phosphorylated in purified virions whereas phosphorylated species of the M and Vp24 proteins were minor components. The phosphorylated form of the P protein was detected by monoclonal antibody precipitation, confirming the identity of this protein. The N protein was not phosphorylated in infected cells or in virions. Synthesis of [35S]methionine-labelled proteins preceded detectable 32Pi labelling by several hours. The putative phosphorylated M protein was detected at 6 h p.i. before phosphorylated forms of P and Vp24 were seen. The timing of appearance of the phosphorylated species of P and Vp24 proteins in infected cells corresponded to the release of infectious virions from infected cell monolayers at 10 to 12 h p.i.
Keywords: RS virus, polypeptide synthesis, kinetics
Received 29 July 1987;
accepted 19 October 1987.
This article has been cited by other articles:
|
|
 |
|
  H. W. McL. Rixon, G. Brown, J. T. Murray, and R. J. Sugrue The respiratory syncytial virus small hydrophobic protein is phosphorylated via a mitogen-activated protein kinase p38-dependent tyrosine kinase activity during virus infection J. Gen. Virol., February 1, 2005; 86(2): 375 - 384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. W. Mason, E. Aberg, C. Lawetz, R. DeLong, P. Whitehead, and M. Liuzzi Interaction between Human Respiratory Syncytial Virus (RSV) M2-1 and P Proteins Is Required for Reconstitution of M2-1-Dependent RSV Minigenome Activity J. Virol., October 1, 2003; 77(19): 10670 - 10676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Weik, J. Modrof, H.-D. Klenk, S. Becker, and E. Muhlberger Ebola Virus VP30-Mediated Transcription Is Regulated by RNA Secondary Structure Formation J. Virol., July 29, 2002; 76(17): 8532 - 8539. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. W. Thomas, M. M. Monick, J. M. Staber, T. Yarovinsky, A. B. Carter, and G. W. Hunninghake Respiratory Syncytial Virus Inhibits Apoptosis and Induces NF-kappa B Activity through a Phosphatidylinositol 3-Kinase-dependent Pathway J. Biol. Chem., January 4, 2002; 277(1): 492 - 501. [Abstract] [Full Text]
|
|
|
|
|
|
T. L. Cartee and G. W. Wertz Respiratory Syncytial Virus M2-1 Protein Requires Phosphorylation for Efficient Function and Binds Viral RNA during Infection J. Virol., December 15, 2001; 75(24): 12188 - 12197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Cuesta, X. Geng, A. Asenjo, and N. Villanueva Structural Phosphoprotein M2-1 of the Human Respiratory Syncytial Virus Is an RNA Binding Protein J. Virol., November 1, 2000; 74(21): 9858 - 9867. [Abstract] [Full Text]
|
|
|
|
|
|
R. W. Hardy and G. W. Wertz The Cys3-His1 Motif of the Respiratory Syncytial Virus M2-1 Protein Is Essential for Protein Function J. Virol., July 1, 2000; 74(13): 5880 - 5885. [Abstract] [Full Text]
|
|
|
|
|
|
N. Villanueva, R. Hardy, A. Asenjo, Q. Yu, and G. Wertz The bulk of the phosphorylation of human respiratory syncytial virus phosphoprotein is not essential but modulates viral RNA transcription and replication J. Gen. Virol., January 1, 2000; 81(1): 129 - 133. [Abstract] [Full Text]
|
|
|
|
|
|
G. Ahmadian, P. Chambers, and A. J. Easton Detection and characterization of proteins encoded by the second ORF of the M2 gene of pneumoviruses J. Gen. Virol., August 1, 1999; 80(8): 2011 - 2016. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
