| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Virology Laboratories, Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205, U.S.A.
We have previously described blocks to the viral lytic cycle at two different levels in cytomegalovirus (CMV)-infected non-permissive cells. BALB/c-3T3 cells express only the predominant immediate early (IE) nuclear phosphoproteins (IE68 or IE94) of human CMV (HCMV) or simian CMV (SCMV) and do not replicate the input viral genomes. However, in human teratocarcinoma stem cells and 293 cells, expression of the HCMV IE68 gene (but not the SCMV IE94 gene) is blocked at the transcriptional level. Here we report the results of an extensive comparison of the level of permissiveness for HCMV, SCMV and murine CMV (MCMV) in a variety of additional cell types of human, monkey and mouse origin. We also describe a subtle change in the tryptic peptide pattern of the IE68 polypeptide produced in BALB/c-3T3 cells compared to permissive human foreskin fibroblasts. Neither the IE68 nor IE94 proteins could be detected by biochemical labelling procedures in infected mouse Ltk- or F9 teratocarcinoma stem cells, although IE94 was synthesized after retinoic acid-induced differentiation of the F9 cells. Synthesis of [35S]methionine-labelled IE94 protein, but not that of HCMV IE68, was detected in infected Vero cells and in human peripheral blood leukocyte cultures. The failure to synthesize detectable IE68 protein in infected Vero cells appeared to be unrelated to a lack of entry of viral DNA and to a lack of appropriate transcription factors. Indeed, immunofluorescence assays showed that the IE68 antigen was expressed efficiently in DNA-transfected Vero cells and in a small fraction of infected Vero cells. Overall, two clear host range trends emerged. First, whilst all three viruses showed a tendency for repression of IE expression in transformed cell lines, the effect was severe for HCMV and only minimal for SCMV. Secondly, progression of infection to the viral DNA synthesis level in non-transformed fibroblast cell types occurred in a much wider range of host species cell types for SCMV and MCMV than for HCMV.
Keywords: CMV, gene expression, DNA replication
Present address: Merck Sharp & Dohme, Virus & Cell Biology Research Laboratories, West Point, Pennsylvania 19486, U.S.A.
Received 20 July 1987;
accepted 6 November 1987.
This article has been cited by other articles:
|
|
 |
|
  L. Wiebusch, A. Neuwirth, L. Grabenhenrich, S. Voigt, and C. Hagemeier Cell Cycle-Independent Expression of Immediate-Early Gene 3 Results in G1 and G2 Arrest in Murine Cytomegalovirus-Infected Cells J. Virol., October 15, 2008; 82(20): 10188 - 10198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. T. Saffert and R. F. Kalejta Human Cytomegalovirus Gene Expression Is Silenced by Daxx-Mediated Intrinsic Immune Defense in Model Latent Infections Established In Vitro J. Virol., September 1, 2007; 81(17): 9109 - 9120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Tang and G. G. Maul Mouse cytomegalovirus crosses the species barrier with help from a few human cytomegalovirus proteins. J. Virol., August 1, 2006; 80(15): 7510 - 7521. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Tavalai, P. Papior, S. Rechter, M. Leis, and T. Stamminger Evidence for a Role of the Cellular ND10 Protein PML in Mediating Intrinsic Immunity against Human Cytomegalovirus Infections. J. Virol., August 1, 2006; 80(16): 8006 - 8018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Tang, E. A. Murphy, and G. G. Maul Experimental Confirmation of Global Murine Cytomegalovirus Open Reading Frames by Transcriptional Detection and Partial Characterization of Newly Described Gene Products J. Virol., July 15, 2006; 80(14): 6873 - 6882. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Sinclair and P. Sissons Latency and reactivation of human cytomegalovirus J. Gen. Virol., July 1, 2006; 87(7): 1763 - 1779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Reinhardt, G. B. Smith, C. T. Himmelheber, J. Azizkhan-Clifford, and E. S. Mocarski The Carboxyl-Terminal Region of Human Cytomegalovirus IE1491aa Contains an Acidic Domain That Plays a Regulatory Role and a Chromatin-Tethering Domain That Is Dispensable during Viral Replication J. Virol., January 1, 2005; 79(1): 225 - 233. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Tang, L. Li, and G. G. Maul Mouse Cytomegalovirus Early M112/113 Proteins Control the Repressive Effect of IE3 on the Major Immediate-Early Promoter J. Virol., January 1, 2005; 79(1): 257 - 263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Adamo, J. Schroer, and T. Shenk Human Cytomegalovirus TRS1 Protein Is Required for Efficient Assembly of DNA-Containing Capsids J. Virol., October 1, 2004; 78(19): 10221 - 10229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Gillet, F. Minner, B. Detry, F. Farnir, L. Willems, M. Lambot, E. Thiry, P.-P. Pastoret, F. Schynts, and A. Vanderplasschen Investigation of the Susceptibility of Human Cell Lines to Bovine Herpesvirus 4 Infection: Demonstration that Human Cells Can Support a Nonpermissive Persistent Infection Which Protects Them against Tumor Necrosis Factor Alpha-Induced Apoptosis J. Virol., March 1, 2004; 78(5): 2336 - 2347. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wang, M. Messerle, R. Sapinoro, K. Santos, P. K. Hocknell, X. Jin, and S. Dewhurst Murine Cytomegalovirus Abortively Infects Human Dendritic Cells, Leading to Expression and Presentation of Virally Vectored Genes J. Virol., July 1, 2003; 77(13): 7182 - 7192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Isomura and M. F. Stinski The Human Cytomegalovirus Major Immediate-Early Enhancer Determines the Efficiency of Immediate-Early Gene Transcription and Viral Replication in Permissive Cells at Low Multiplicity of Infection J. Virol., March 15, 2003; 77(6): 3602 - 3614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Ellsmore, G. G. Reid, and N. D. Stow Detection of human cytomegalovirus DNA replication in non-permissive Vero and 293 cells J. Gen. Virol., March 1, 2003; 84(3): 639 - 645. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. C. Erlach, J. Podlech, A. Rojan, and M. J. Reddehase Tumor Control in a Model of Bone Marrow Transplantation and Acute Liver-Infiltrating B-Cell Lymphoma: an Unpredicted Novel Function of Cytomegalovirus J. Virol., February 22, 2002; 76(6): 2857 - 2870. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xu, J.-H. Ahn, M. Cheng, C. M. apRhys, C.-J. Chiou, J. Zong, M. J. Matunis, and G. S. Hayward Proteasome-Independent Disruption of PML Oncogenic Domains (PODs), but Not Covalent Modification by SUMO-1, Is Required for Human Cytomegalovirus Immediate-Early Protein IE1 To Inhibit PML-Mediated Transcriptional Repression J. Virol., November 15, 2001; 75(22): 10683 - 10695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Sandford, L. E. Brock, S. Voigt, C. M. Forester, and W. H. Burns Rat Cytomegalovirus Major Immediate-Early Enhancer Switching Results in Altered Growth Characteristics J. Virol., June 1, 2001; 75(11): 5076 - 5083. [Abstract] [Full Text]
|
|
|
|
|
|
J.-H. Ahn, Y. Xu, W.-J. Jang, M. J. Matunis, and G. S. Hayward Evaluation of Interactions of Human Cytomegalovirus Immediate-Early IE2 Regulatory Protein with Small Ubiquitin-Like Modifiers and Their Conjugation Enzyme Ubc9 J. Virol., April 15, 2001; 75(8): 3859 - 3872. [Abstract] [Full Text]
|
|
|
|
|
|
J. J. García-Ramírez, F. Ruchti, H. Huang, K. Simmen, A. Angulo, and P. Ghazal Dominance of Virus over Host Factors in Cross-Species Activation of Human Cytomegalovirus Early Gene Expression J. Virol., January 1, 2001; 75(1): 26 - 35. [Abstract] [Full Text]
|
|
|
|
|
|
A. Angulo, M. Messerle, U. H. Koszinowski, and P. Ghazal Enhancer Requirement for Murine Cytomegalovirus Growth and Genetic Complementation by the Human Cytomegalovirus Enhancer J. Virol., November 1, 1998; 72(11): 8502 - 8509. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-H. Ahn, E. J. Brignole III, and G. S. Hayward Disruption of PML Subnuclear Domains by the Acidic IE1 Protein of Human Cytomegalovirus Is Mediated through Interaction with PML and May Modulate a RING Finger-Dependent Cryptic Transactivator Function of PML Mol. Cell. Biol., August 1, 1998; 18(8): 4899 - 4913. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
