HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Barron, C. Articles by Bade, E. G. Search for Related Content PubMed PubMed Citation Articles by Barron, C. Articles by Bade, E. G. Agricola Articles by Barron, C. Articles by Bade, E. G.

Transcriptional Mapping of the Bacteriophage Mu DNA

Carlos Barron

Fakultät für Biologie, Universität Konstanz, Postfach 7733, 7750 Konstanz, F.R.G.

The transcription of temperate phage Mu throughout lytic development was analysed quantitatively by hybridization of pulse-labelled RNA to full-length Mu DNA and to plasmids that define Mu DNA segments covering the whole phage genome. The transcription rate (i.e. binding data corrected for the incorporation rate of the radioactive precursor, for the size of the DNA template, and for the number of phage genomes present in the bacterium at the time of analysis) revealed three defined phases of Mu transcription: early (0 to 9 min), intermediate (between 9 and the interval 14 to 17 min) and late (from the interval 14 to 17 min onward). The analysis also revealed that the region comprising the genes involved in phage morphogenesis was organized into two independent ‘late’ transcription units.

Keywords: phage Mu, transcription rate, RNA-DNA hybridization

Present address: Max-Planck-Institut für Biochemie, 8033 Martinsried bei München, F.R.G.

Received 9 February 1987; accepted 10 September 1987.