J Gen Virol Try IJSEM Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 69 (1988), 401-406; DOI 10.1099/0022-1317-69-2-401
© 1988 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Russo, M.
Right arrow Articles by Morris, T. J.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Russo, M.
Right arrow Articles by Morris, T. J.
Agricola
Right arrow Articles by Russo, M.
Right arrow Articles by Morris, T. J.

Complementary DNA Cloning and Characterization of Cymbidium Ringspot Virus RNA

Marcello Russo1, Jòzsef Burgyan1, James C. Carrington2, Bradley I. Hillman2 and Thomas J. Morris2

1 Dipartimento di Patologia Vegetale, Università degli Studi and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, Via Amendola, 165/A 70125 Bari, Italy
and2 Department of Plant Pathology, University of California, Berkeley, California 94702, U.S.A.

DNA complementary to the 4.7 kb cymbidium ringspot virus (CyRSV) genome was cloned in Escherichia coli. Recombinant plasmids were screened with a cDNA probe synthesized from randomly primed CyRSV genomic RNA, and two clones were chosen for further analysis. Two additional subclones were prepared after deletion of two sequences in the 3' region and one clone containing 5' virus genome sequences was constructed by primer extension. Three different RNA species associated with CyRSV were detected in purified virions and virus-infected tissue. The largest RNA was genomic RNA and the other two subgenomic. Northern blot hybridization indicated that the two subgenomic RNAs had in common a region corresponding to the 3' region of genomic RNA. All three RNAs were found in tissue in both single- and double-stranded forms. In vitro translation experiments with fractionated virus RNA indicated that the coat protein gene is internally located and that it is followed by a 3'-terminal gene coding for a 22K protein.

Keywords: CyRSV, cDNA cloning, RNA characterization

Received 20 July 1987; accepted 29 October 1987.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1988 by the Society for General Microbiology.