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Infection by Rice Gall Dwarf Virus of Cultured Monolayers of Leafhopper Cells

¹ National Agriculture Research Center
² National Institute of Agrobiological Resources, Tsukuba Science City, Yatabe, Ibaraki 305, Japan
and³ Food & Fertilizer Technology Center for the Asian and Pacific Region, Taipei, Taiwan

Synchronous multiplication of rice gall dwarf virus (RGDV) and an assay of its infectivity in cell monolayers of its vector, the green rice leafhopper Nephotettix cincticeps, are described. The number of foci of infected cells was linearly related to the concentration of virus. The method was about 10³ times more sensitive than enzymelinked immunosorbent assay. All the vector cells in monolayers were infected when inocula were dilutions of 10^-3.5 of sap from infected plants, 10^-4.5 of extracts of viruliferous insects, 10^-5.5 of infected monolayer cells or purified virus with A = 10^-5. RGDV was first detected in monolayer cells 10 h after inoculation, and multiplied 10⁵-fold in the subsequent 40 h. Vector cell monolayers are thus an excellent experimental system for the study of RGDV.

Keywords: RGDV, phytoreovirus, insect cell monolayers

Received 6 May 1987; accepted 29 September 1987.

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				T. Wei, T. Uehara-Ichiki, N. Miyazaki, H. Hibino, K. Iwasaki, and T. Omura Association of Rice Gall Dwarf Virus with Microtubules Is Necessary for Viral Release from Cultured Insect Vector Cells J. Virol., October 15, 2009; 83(20): 10830 - 10835. [Abstract] [Full Text] [PDF]