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Isolation of a Simian Virus 40-Simian Agent 12 Recombinant Papovavirus That Synthesizes a Hybrid Large Tumour Antigen

Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, U.S.A.

The simian virus 40 (SV40) mutant dl1055 carries a 55 base pair deletion in the viral early region coding sequences that causes premature termination of large tumour (T) antigen translation resulting in a protein fragment consisting of the amino-terminal 399 residues. The mutation renders the virus defective. We report the characterization of hyb4001, which was isolated as a single plaque following cotransfection of permissive cells with dl1055 DNA and the DNA of a related papovavirus, simian agent 12 (SA12). Hyb4001 arose by two homologous recombination events involving crossovers in regions of 7 and 12 base pairs of perfect homology between the two viruses. Hyb4001 synthesizes a hybrid T antigen with the amino-terminal 382 residues encoded by SV40, residues 383 to 449 encoded by SA12, and the carboxy-terminal 259 residues encoded by SV40.

Keywords: SV40-SA12 recombinant, T antigen, papovaviruses

Received 16 September 1987; accepted 16 November 1987.