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J Gen Virol 69 (1988), 699-704; DOI 10.1099/0022-1317-69-3-699
© 1988 Society for General Microbiology
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Analysis and Isolation of Cytomegalovirus DNA by Field Inversion Gel Electrophoresis

Frank M. Van Den Berg1, Mehdi Jiwa1, Rien Rook1 and Jan L. M. C. Geelen2

1 Department of Pathology
and2 Department of Virology, Academic Medical Centre, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands

High molecular weight human cytomegalovirus (CMV) DNA was isolated from agarose-embedded infected human diploid cells by employing field inversion gel electrophoresis. A high yield of CMV DNA molecules was obtained within 1 week of infecting the cell culture. Labelling of the viral DNA with biotin by nick translation enabled the detection of CMV-infected cells in sections of paraffin-embedded human adrenal gland by in situ hybridization.

Keywords: CMV DNA, FIGE, in situ hybridization

Received 11 August 1987; accepted 9 November 1987.




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