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A 1.9 Kilobase Homology in the 3'-terminal Regions of RNA-1 and RNA-2 of Tomato Ringspot Virus

¹ Department of Plant Science, University of British Columbia, Vancouver, British Columbia V6T 2A2
and² Agriculture Canada, Research Station, 6660 N.W. Marine Drive, Vancouver, British Columbia V6T 1X2, Canada

Double-stranded cDNA copies of RNA-1 and RNA-2 of tomato ringspot virus were cloned into pUC9. Comparison of restriction enzyme maps of eight clones indicated that 1.9 kb at the 3' termini of RNA-1 and RNA-2 were similar. Five of these clones were used in Northern hybridization analyses and found to contain sequences either unique to RNA-1 or RNA-2, or common to RNA-1 and RNA-2. Southern blot analyses using clones derived from RNA-1 and RNA-2 confirmed that there is a 1.9 kb nucleotide sequence homology at the 3' termini. A sequence homology of this magnitude has not been reported previously for other plant viruses with multipartite ssRNA genomes.

Keywords: TomRSV, sequence homology, 3'-terminal sequences

Received 12 October 1987; accepted 9 December 1987.