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Detection of Viral Proteins in Cytopathic Structures in Cowpea Protoplasts Infected with Cowpea Mosaic Virus

Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC Wageningen
and¹ Department of Virology, Binnenhaven 11, 6709 PD Wageningen, The Netherlands

Infection of cowpea (Vigna unguiculata) cells or protoplasts with cowpea mosaic virus is accompanied by the appearance of characteristic cytopathic structures. A major constituent of these cytopathic structures, represented in thin sections by electrondense material, was shown to contain viral non-structural proteins by its reaction with antisera to non-structural proteins and their detection with colloidal gold-labelled Protein A. With this same technique virus particles were found to accumulate mainly in the cytoplasm.

Keywords: CPMV, cytopathic structures, immunogold labelling

Received 7 September 1987; accepted 3 December 1987.