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Cell-free Translation of Maize Rayado Fino Virus Genomic RNA

Ana M. Espinoza

Centro de Investigación en Biología Celular y Molecular, Universidad de Costa Rica, Ciudad Universitaria 2060, Costa Rica

In reticulocyte lysates maize rayado fino virus RNA directed the synthesis of a large number of polypeptides ranging in M_r from 15000 to 165000. Partial proteolysis showed that many of the larger polypeptides contained the same peptides. When tRNA was added to the lysate proportionally more of the large M_r products were produced. No polypeptides were detected that comigrated with coat proteins and no coat protein was detected in translation products by ELISA or by immunoprecipitation. We found little evidence of polyprotein degradation among the translation products and no evidence for encapsidated subgenomic mRNAs for the capsid polypeptides.

Keywords: MRFV, translation in vitro, genomic RNA

Present address: John Innes Institute, Colney Lane, Norwich NR4 7UH, U.K.

Received 22 September 1987; accepted 14 December 1987.