HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ebner, D. Articles by Siddell, S. G. Search for Related Content PubMed PubMed Citation Articles by Ebner, D. Articles by Siddell, S. G. Agricola Articles by Ebner, D. Articles by Siddell, S. G.

Identification of the Coronavirus MHV-JHM mRNA 4 Product

Institute of Virology, University of Würzburg, Versbacherstrasse 7, 8700 Würzburg, F.R.G.

A bacterial expression vector was constructed to encode a fusion protein which had, at its carboxy terminus, a polypeptide encoded within the 5' proximal open reading frame of the coronavirus MHV-JHM mRNA 4. This polypeptide was isolated and used to produce an antiserum. The antiserum reacted specifically with a 15000 M_r polypeptide synthesized in MHV-JHM-infected cells, or in vitro translations of infected cell poly(A) RNA enriched for mRNA 4. These results demonstrate the translational activity of mRNA 4 during infection, identify conclusively the translation product and provide a means to investigate the synthesis and function of this protein.

Keywords: coronavirus MHV, mRNA 4, translation product

Received 23 September 1987; accepted 1 February 1988.