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Protein Coding Assignment for the Genome of Epizootic Haemorrhagic Disease Virus

Agricultural Research Service, Arthropod-borne Animal Diseases Research Laboratory, P.O. Box 3965, University Station, Laramie, Wyoming 82071-3965, U.S.A.

Viral genomic RNA was purified from BHK-21 cells infected with epizootic haemorrhagic disease virus and the 10 dsRNA genome segments were isolated by polyacrylamide gel electrophoresis. These genome segments were translated in vitro using the rabbit reticulocyte lysate system and the synthesized proteins were detected by immune precipitation and gel electrophoresis. This allowed the assignment of protein coding to the genome segments and the identification of two additional virus-specified proteins not readily detectable in lysates of virus-infected cells.

Keywords: EHDV, dsRNA, translation

Received 9 November 1987; accepted 18 March 1988.

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