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J Gen Virol 69 (1988), 1285-1298; DOI 10.1099/0022-1317-69-6-1285
© 1988 Society for General Microbiology
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Physical Maps and Comparative DNA Hybridization of Mamestra Brassicae and Panolis Flammea Nuclear Polyhedrosis Virus Genomes

R. D. Possee and D. C. Kelly{dagger}

NERC Institute of Virology, Mansfield Road, Oxford OX1 3SR, U.K.

The genome DNAs from the Panolis flammea (Pf) multiple nucleocapsid nuclear polyhedrosis virus (MNPV) and the Mamestra brassicae (Mb) MNPV were analysed with the restriction endonucleases BamHI, BglII, KpnI, HindIII, SmaI and XhoI. The profiles produced by each enzyme for the two virus genomes were quite dissimilar with very few comigrating fragments. The size of PfMNPV DNA was calculated to be 145 kilobase pairs (kbp) and that of MbMNPV 150 kbp. Physical maps of the two genomes were constructed utilizing the above enzymes. The two maps were oriented in relation to their putative polyhedrin genes. Alignment of the two restriction maps for PfMNPV and MbMNPV was achieved by performing cross blot hybridization between XhoI digests of the two virus genomes. This showed that despite differences in the physical maps the two genomes shared overall similarity in gene organization. The two viruses were also compared using dot blot hybridization analysis to quantify homology with Autographa californica (Ac) MNPV. These data showed that both PfMNPV and MbMNPV were distantly related to AcMNPV but exhibited a high degree of homology to each other (nearly 100% in 20% formamide, 70% in 50% formamide).

Keywords: baculovirus DNA, physical maps, DNA hybridization

{dagger} Present address: Chemical Defence Establishment, Porton Down, Salisbury, Wiltshire SP4 0JQ, U.K.

Received 30 September 1987; accepted 16 February 1988.




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Copyright © 1988 by the Society for General Microbiology.