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Characterization of a Subgenomic DNA Isolated from Triticum Aestivum Plants Infected with Wheat Dwarf Virus

H. Macdonald

Department of Pure and Applied Biology, Imperial College of Science and Technology, London SW7 2BB, U.K.

A subgenomic DNA has been isolated from wheat tissue infected with a Swedish isolate of wheat dwarf virus and cloned. Restriction endonuclease analysis and nucleotide sequence determination indicated that the subgenomic DNA (1472 nucleotides) was derived from the genomic DNA (2749 nucleotides) by two separate deletions. The subgenomic DNA had lost open reading frames (ORFs) encoding the virus coat protein and a putative protein of M_r 10146, but retained an ORF and an open reading region encoding putative proteins of M_r 30156 and 17292, respectively, and two structural features thought to be important for virus DNA replication i.e. a potentially stable stem-loop structure containing the conserved TAATATTAC sequence and a putative primer initiation site.

Keywords: WDV, subgenomic DNA, replication

Present address: Friedrich Miescher-Institut, P.O. Box 2543, CH-4002 Basel, Switzerland.

Received 22 December 1987; accepted 26 February 1988.