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Priming of Complementary DNA Synthesis in Vitro by Small DNA Molecules Tightly Bound to Virion DNA of Wheat Dwarf Virus

H. Macdonald

Department of Pure and Applied Biology, Imperial College of Science and Technology, London SW7 2BB, U.K.

DNA isolated from purified preparations of wheat dwarf virus (WDV) has been shown to contain tightly bound small DNA molecules which can act as primers for the synthesis of full-length complementary DNA in vitro. The small DNA molecules are bound in the terminating intergenic region of the WDV genome between the end of an open reading frame encoding a putative protein of M_r 17292 and a conserved ‘A-T’ box containing putative transcriptional polyadenylation signals. Evidence that the small DNA molecules contain ribonucleotides at their 5' termini is presented and their possible role in the priming of virus DNA synthesis in vivo is discussed.

Keywords: WDV, geminivirus, priming

Present address: Friedrich Miescher-Institut, P.O. Box 2543, CH-4002 Basel, Switzerland.

Received 20 October 1987; accepted 26 February 1988.