J Gen Virol Try Microbiology Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 69 (1988), 1367-1373; DOI 10.1099/0022-1317-69-6-1367
© 1988 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Moser, O.
Right arrow Articles by Mundry, K.-W.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Moser, O.
Right arrow Articles by Mundry, K.-W.
Agricola
Right arrow Articles by Moser, O.
Right arrow Articles by Mundry, K.-W.

The Fate of the Transport Protein of Tobacco Mosaic Virus in Systemic and Hypersensitive Tobacco Hosts

O. Moser1, M.-J. Gagey1, T. Godefroy-Colburn1, C. Stussi-Garaud1, M. Ellwart-Tschürtz2, H. Nitschko2 and K.-W. Mundry2

1 Institut de Biologie Moléculaire des Plantes du Centre National de la Recherche Scientifique, 12 rue du Général Zimmer, 67000 Strasbourg, France
and2 Biologisches Institut der Universität Stuttgart, Ulmerstrasse 227, D-7000 Stuttgart 60, F.R.G.

The transport protein of tobacco mosaic virus (TMV) (Mr 30000, 30K non-structural protein) was detected on Western blots, using an antiserum to a synthetic C-terminal nonapeptide. Accumulation of this protein in subcellular fractions of inoculated leaves was measured during TMV infection of Nicotiana tabacum cv. Samsun and cv. Samsun NN. In cv. Samsun, a systemic host, the 30K protein appeared transiently in a crude membrane fraction but accumulated more stably in cell walls. In cv. Samsun NN, which is a hypersensitive host giving only localized infection, the early accumulation (up to 40 h; before any necrosis was visible) was the same as in cv. Samsun. However, as soon as necrosis was visible, the amount of 30K detected in the cell wall fraction decreased sharply and coat protein synthesis stopped. This drop in the amount of 30K protein is most easily interpreted as a side-effect of the hypersensitive reaction and may explain why TMV infection becomes localized in leaves of cv. Samsun NN.

Keywords: TMV, virus transport, hypersensitive reaction

Received 4 December 1987; accepted 18 February 1988.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1988 by the Society for General Microbiology.