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The Genome of the Multicapsid Baculovirus of Orgyia Pseudotsugata: Restriction Map and Analysis of Two Sets of GC-rich Repeated Sequences

Department of Agricultural Chemistry, Oregon State University, Corvallis, Oregon 97331, U.S.A.

Five cosmids containing inserts that comprise the complete genome of the multicapsid nuclear polyhedrosis virus of Orgyia pseudotsugata were mapped with four restriction enzymes (BglII, ClaI, SstI, XhoI). From these cosmid maps, composite maps of the complete genome were constructed for each restriction enzyme. A region containing repeats of the sequence GGC downstream of the polyhedrin gene was used to probe the genome. It cross-hybridized with a region which, upon sequence analysis, was found to be a highly repetitive GC-rich region of nearly 500 nucleotides. The two GC-rich regions appeared to be evolutionarily unrelated.

Keywords: OpMNPV, genome map, baculovirus

Received 30 September 1987; accepted 16 February 1988.