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J Gen Virol 69 (1988), 1741-1747; DOI 10.1099/0022-1317-69-7-1741
© 1988 Society for General Microbiology
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Physical Maps of the Genomes of Four Variants of Artogeia rapae Granulosis Virus

Ian R. L. Smith{dagger} and Norman E. Crook

AFRC Institute of Horticultural Research, Worthing Road, Littlehampton, West Sussex BN17 6LP, U.K.

The genomes of four closely related variants of Artogeia rapae granulosis virus, isolated in England and New Zealand, have been mapped for the restriction endonucleases EcoRI, BamHI, HindIII, KpnI, XhoI, PstI, BglI, and SmaI by secondary digestion of isolated restriction fragments. A total of 69, 70 or 71 fragments were unambiguously mapped for these isolates. A modified system of fragment lettering was adopted so that, for each enzyme, the same letter could be used for colinear fragments from each isolate despite loss or gain of restriction sites. The sizes of the viral DNAs varied slightly, but were all about 110 kbp. The zero point for the maps was taken as the start of the granulin gene which was orientated in the same direction as the polyhedrin gene on the map of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus DNA. Differences between the four variants are discussed in relation to their insect host range.

Keywords: baculovirus, host range, Artogeia rapae, genome maps

{dagger} Present address: Boyce Thompson Institute for Plant Research, Cornell University, Tower Road, Ithaca, New York 14853, U.S.A.

Received 7 December 1987; accepted 28 March 1988.




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