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J Gen Virol 69 (1988), 1931-1939; DOI 10.1099/0022-1317-69-8-1931
© 1988 Society for General Microbiology
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Stable Expression of the Hepatitis B Virus Surface Antigen Containing Pre-S2 Protein in Mouse Cells Using a Bovine Papillomavirus Vector

Tetsuo Yoneyama, Toshitaka Akatsuka and Tatsuo Miyamura

Department of Enteroviruses, National Institute of Health, 10-35, Kamiosaki, 2-Chome, Shinagawa-ku, Tokyo 141, Japan

The large Bg/II fragment (2.8 kilobases) of hepatitis B virus DNA including the transcription unit for the hepatitis B surface antigen (HBsAg) was inserted into a bovine papillomavirus vector containing the neomycin resistance gene. The recombinant DNA was transfected into mouse C127 cells. A stable transformed cell line (MS128) secreting a large amount of 22 nm HBsAg particles containing pre-S2 protein was established. The secreted HBsAg particles had the receptor for polymerized human serum albumin. Immunoprecipitation and Western blot analyses showed that HBsAg particles consisted of two major proteins of 22K and 26K encoded by the S gene and a minor protein of 35K encoded by the pre-S2 and S genes. Southern blot analysis revealed that the transfected plasmid was integrated into the host chromosomal DNA and that most of the plasmid sequences were present. These results suggest that the stable expression of the HBsAg in MS128 cells is related to the integrated state of the recombinant DNA.

Keywords: HBsAg, BPV vector, integration, pre-S2 protein

Received 24 February 1988; accepted 10 May 1988.




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Copyright © 1988 by the Society for General Microbiology.