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J Gen Virol 69 (1988), 2277-2289; DOI 10.1099/0022-1317-69-9-2277
© 1988 Society for General Microbiology
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DNA-binding Proteins Induced by the Cottontail Rabbit Herpesvirus CTHV

C. Cajean-Feroldi, M. Laithier, T. Foulon and P. Sheldrick

Institut de Recherches Scientifiques sur le Cancer, B.P. no. 8, 94802 Villejuif Cedex, France

Several new polypeptides were detected in cells infected with CTHV, a cottontail rabbit herpesvirus. All of them (Mr 150K, 110K, 93K, 83K, 75K and 35K) accumulated in the nucleus during the infectious cycle, and all except the 150K species bound to DNA-cellulose affinity columns in low-salt buffers. Polyclonal antisera prepared against the 35K DNA-binding protein also recognized the 75K species. Although the 75K protein could be detected earlier in infection than the 35K protein, late in the infectious cycle the latter increased to an abundance approaching that of cellular histones. Treatment of partially purified virions with a non-ionic detergent indicated that the 35K protein, but not the 75K protein, is a component of capsid/tegument structures. The anti-35K/75K serum did not cross-react with herpesvirus sylvilagus virion proteins, which, in an electrophoretic comparison, exhibited both similarities to and differences from the virion proteins of CTHV. Labelling of CTHV-infected cells with [32P]orthophosphate revealed the presence of phosphoproteins electrophoretically comigrating with the 93K, 83K, 75K and 35K proteins.

Keywords: CTHV, DNA-binding proteins, phosphoproteins

Received 4 January 1988; accepted 2 June 1988.




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Copyright © 1988 by the Society for General Microbiology.