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Molecular Properties of Bari 1, a Mild Strain of Cauliflower Mosaic Virus

Department of Virus Research, John Innes Institute, AFRC Institute of Plant Science Research, Colney Lane, Norwich NR4 7UH, U.K.

We studied aspects of the structure and expression of the genome of Bari 1, a mild strain of cauliflower mosaic virus. Differences were observed between gene products of Bari 1 detected in inclusion body preparations and those of the more typically severe strain, Cabb B-JI. The most striking difference was the gel mobility of the Bari 1 gene VI polypeptide (apparent M_r 70K) which contrasted with that of Cabb B-JI (M_r 62K). This difference was also observed between products of in vitro translation of viral mRNA suggesting that it was not due to post-translational modification. The open reading frame in the nucleotide sequence of the Bari 1 gene VI region was very similar in size to that of other CaMV strains but corresponded to an amino acid sequence with a much lower overall homology and diverged greatly in a 40 base pair sequence in the 3' region compared to gene VI sequences of other strains. The level of the Bari 1 aphid transmission polypeptide P18, the product of gene II, was much lower than that of Cabb B-JI. Some of the possible subcellular consequences resulting from the molecular properties of Bari 1 were examined by electron microscopy. Differences were observed in the composition and intactness of Bari 1 cytoplasmic inclusion bodies compared with those of a severe strain, and the presence of nuclear inclusions.

Keywords: CaMV, genome, inclusion bodies

Received 5 February 1988; accepted 20 May 1988.