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Isolation and Characterization of Two Plaque Size Variants of Theiler's Murine Encephalomyelitis Virus (DA Strain)

¹ Department of Pathology and Laboratory Medicine, University of Texas Medical School at Houston, 6431 Fannin, Houston, Texas 77030
and² Departments of Neurology and Immunology, Mayo Medical School, 200 First Street S.W., Rochester, Minnesota 55905, U.S.A.

We have isolated two plaque size variants of Theiler's murine encephalomyelitis virus (TMEV) strain DA. One variant, TMEV-C_L (C_L), produced large plaques, while the other, TMEV-D_S (D_S), produced small plaques in L-2 cells. The D_S variant yielded a lower titre in BHK cells and had a significantly slower growth rate when compared to C_L and DA. In contrast, D_S replicated to a higher titre in the central nervous system (CNS) of infected mice than the large plaque counterpart and DA. Furthermore, the D_S (but not C_L) variant was temperature-sensitive, replicating 130- to 500-fold more at 37 °C than at 39 °C. Although D_S, C_L and DA were able to establish persistent CNS infections in mice, only the D_S variant and DA induced demyelinating disease in SJL/J mice. Therefore persistence of TMEV in the CNS is not sufficient to produce demyelinating disease. These two variants of the DA strain of TMEV will be useful for study of the viral genetic elements important in the mechanism of virus-induced demyelination.

Keywords: TMEV, plaque variants, demyelination

Received 11 March 1988; accepted 25 May 1988.

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