HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Martin, S. J. Articles by Clements, J. B. Search for Related Content PubMed PubMed Citation Articles by Martin, S. J. Articles by Clements, J. B. Agricola Articles by Martin, S. J. Articles by Clements, J. B.

Purification and Characterization of Bovine Enteroviruses

Department of Biochemistry, Queen's University, Belfast, N. Ireland

Three serologically distinct bovine enteroviruses (VG-5-27; VC-65-182; VP-7-19) grew in BHK21 cells to concentrations of approximately 5 x 10⁸ p.f.u./ml. A purification procedure was used which involved sedimentation and gel-filtration through a Sepharose 2 B column. The virus particles were spherical (diameter of 27 nm.), had a buoyant density of 1.34 g./ml. in CsCl and a sedimentation coefficient of approximately 165s. The RNA of each of the virus types had a sedimentation coefficient of 35S in sucrose-gradients and the base compositions were similar, being characterized by high A (30%) and low U (21%).

Received 19 September 1969; accepted 19 December 1969.

This article has been cited by other articles:

				M S Smyth and J H Martin Picornavirus uncoating Mol. Pathol., August 1, 2002; 55(4): 214 - 219. [Abstract] [Full Text] [PDF]

				M. Doherty, D. Todd, N. McFerran, and E. M. Hoey Sequence analysis of a porcine enterovirus serotype 1 isolate: relationships with other picornaviruses J. Gen. Virol., August 1, 1999; 80(8): 1929 - 1941. [Abstract] [Full Text]