HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Cartwright, B. Articles by Brown, F. Search for Related Content PubMed PubMed Citation Articles by Cartwright, B. Articles by Brown, F. Agricola Articles by Cartwright, B. Articles by Brown, F.

The Proteins of Biologically Active Sub-units of Vesicular Stomatitis Virus

The Animal Virus Research Institute, Pirbright, Woking Surrey, England

Our experiments on the stepwise degradation of vesicular stomatitis virus into well characterized structural components have enabled us to relate some of the biological properties of the virus to certain of its structural units (Cartwright, Smale & Brown, 1969, 1970). For example, incubating the virus with trypsin produces a bullet-shaped structure similar to the virus except that it no longer possesses the surface projections. Unlike the virus, the projection-free component has low infectivity and does not produce neutralizing antibodies when inoculated into guinea-pigs, thus providing decisive evidence that the immunizing activity of the virus is associated with the surface projections.

By treating the virus with Tween + ether or Nonidet P40 or sodium deoxycholate (Brown, Cartwright & Smale, 1967; Cartwright et al. 1970), the immunizing antigen can be released in a biologically active form, sedimenting at about 6s. An infective skeleton-like structure with the same size and shape as the virus is also produced by treating the virus with Tween + ether or with Nonidet.

Received 2 February 1970; accepted 12 February 1970.