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Temperature-sensitive Mutants of Bovine Herpesvirus Type 1: Mutants Which Make Unaltered Levels of ‘Early’ Glycoproteins but Fail to Synthesize a ‘Late’ Glycoprotein

Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 0W0, Canada

The major glycoproteins of bovine herpesvirus type 1 showed distinct temporal patterns of expression. The glycoproteins GVP 11 and GVP 6 as well as its cleavage products, GVP 11a and GVP 16, were expressed early in the infectious process, whereas GVP 9 was expressed late. Temperature-sensitive mutants were developed and characterized. Mutants belonging to two complementation groups were unable to synthesize DNA at 40 °C, the non-permissive temperature. In cells infected with these mutants the late glycoprotein GVP 9 was not synthesized at 40 °C, whereas the synthesis of the early glycoproteins GVP 11 and GVP 6 continued at wild-type levels. These studies suggest that the transition from early to late glycoprotein synthesis is linked to viral DNA synthesis.

Keywords: BHV-1, temperature-sensitive mutants, glycoproteins, early, late

Received 20 April 1988; accepted 13 October 1988.