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Cytoplasmic Polyhedrosis Virus Classification by Electropherotype; Validation by Serological Analyses and Agarose Gel Electrophoresis

Christopher C. Payne^2,

¹ AFRC Institute for Animal Health, Pirbright Laboratory, Pirbright, Woking, Surrey GU24 0NF
² AFRC Institute of Horticultural Research, Littlehampton, West Sussex BN17 6LP, U.K.
and³ Medical School, University of Cape Town, 7925 Cape Town, South Africa

Serological analyses of several different cytoplasmic polyhedrosis viruses (CPVs), including two type 1 CPVs from Bombyx mori, type 1 CPV from Dendrolimus spectabilis, type 12 CPV from Autographa gamma, type 2 CPV from Inachis io, type 5 CPV from Orgyia pseudotsugata and type 5 CPV from Heliothis armigera, demonstrated a close correlation between the antigenic properties of the polyhedrin or virus particle structural proteins and the genomic dsRNA electropherotypes. The dsRNAs of these viruses were analysed by electrophoresis in 3% and 10% polyacrylamide gels with a discontinuous Tris-HCl/Tris-glycine buffer system or by 1% agarose gel electrophoresis using a continuous Tris-acetate-EDTA buffer system. Electrophoretic analysis in agarose gels was found to be the most suitable for the classification of CPV isolates into electropherotypes, and the results obtained showed a close correlation with the observed antigenic relationships between different virus isolates. However, electrophoretic analysis in 10% polyacrylamide gels was most sensitive for the detection of intra-type variation and the presence of mixed virus isolates.

Keywords: CPV, electropherotype, serological analyses

Present address: AFRC Institute of Horticultural Research, East Malling, Maidstone ME19 6BJ, U.K.

Received 22 June 1988; accepted 26 September 1988.

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