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J Gen Virol 70 (1989), 223-228; DOI 10.1099/0022-1317-70-1-223
© 1989 Society for General Microbiology
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The Large Viral RNA Segment of California Serogroup Bunyaviruses Encodes the Large Viral Protein

Michael J. Endres1, David R. Jacoby1, Robert S. Janssen1,2,{dagger}, Francisco Gonzalez-Scarano1,2, and Neal Nathanson1

1 Department of Microbiology
and2 Department of Neurology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, U.S.A.

Reassortant bunyaviruses derived from two members of the California serogroup (La Crosse/original and Tahyna/181-57) viruses were used to demonstrate that the large Mr viral protein (L) is encoded by the L RNA segment. Radiolabelled viral proteins were analysed by discontinuous SDS-PAGE. The L protein of La Crosse virus was observed to migrate ahead of its Tahyna virus counterpart when electrophoresed through a 5% acrylamide resolving gel. Among the reassortant viruses, the L protein phenotype segregated with the viral L RNA segment. After confirming the genotype of the viruses used in this study, it was concluded that the L RNA species of California serogroup viruses codes for the L protein, the presumed viral polymerase.

Keywords: bunyaviruses, L protein, California serogroup

{dagger} Present address: Centers for Disease Control, Atlanta, Georgia 30333, U.S.A.

Received 18 August 1988; accepted 7 October 1988.


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