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Nucleotide Sequence of the 3'-terminal Region of Potato Virus Y^N RNA

Agricultural University, Department of Virology, Binnenhaven 11, 6709 PD Wageningen, The Netherlands

The sequence of the 3'-terminal 1611 nucleotides of the genome of the tobacco veinal necrosis strain of potato virus Y (PVY^N) was determined. The sequence revealed an open reading frame of 1285 nucleotides, of which the start was not identified, and an untranslated region of 316 nucleotides upstream of a poly(A) tract. Comparison of the open reading frame with the amino-terminal sequence of the viral coat protein enabled mapping of the start of the coat protein at amino acid -267, and indicated that maturation of this protein requires proteolytic processing from a larger polyprptein precursor at a glutamine/glycine dipeptide sequence. The coat protein of PVY^N displayed significant (51 to 63%) sequence homology to the coat proteins of four other potyviruses, tobacco etch virus, tobacco vein mottling virus, plum pox virus and sugarcane mosaic virus. Even higher sequence homology (91%) was detected with the coat protein of a fifth potyvirus, pepper mottle virus (PeMV). This homology was of the same level as found between the coat proteins of PVY^N and a second strain of this virus, PVY^D. Since, moreover, PVY^N and PeMV were the only potyviruses displaying homology in the 3'-terminal, non-translated regions of their genomes, we conclude that PeMV should be regarded as a strain of PVY.

Keywords: PVY^N, potyviruses, PeMV

Received 11 July 1988; accepted 28 September 1988.