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A Defective Interfering RNA Molecule in Cymbidium Ringspot Virus Infections

¹ Research Institute for Plant Protection, P.O. Box 102, H-1525 Budapest, Hungary
and² Dipartimento di Patologia Vegetale, Via Amendola 165/A, Università degli Studi and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, 70126 Bari, Italy

A small RNA (DI RNA), approx. 400 bp in length, was found in plants inoculated with cymbidium ringspot tombusvirus (CyRSV). DI RNA contained sequences derived from at least three regions of the CyRSV genome and was encapsidated in CyRSV protein. DI RNA was formed in plants inoculated with purified genome-size RNA and a double-stranded form was detected in dsRNA extracted from infected tissue. Symptoms of CyRSV infection in Nicotiana clevelandii were less severe when DI RNA was present in the inocula used suggesting that DI RNA interferes with symptom expression.

Keywords: CyRSV, defective interfering RNA

Received 6 June 1988; accepted 28 September 1988.