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Epitope Mapping of Foot-and-Mouth Disease Virus with Neutralizing Monoclonal Antibodies

¹ Wellcome Biotechnology Limited, Langley Court, Beckenham, Kent BR3 3BS
and² Coopers Animal Health Limited, Ash Road, Pirbright, Woking, Surrey GU24 0NQ, U.K.

Epitopes of strain A₂₂ Iraq 24/64 of foot-and-mouth disease virus have been mapped with monoclonal antibodies (MAbs). Three methods were used: (i) an indirect ELISA using an overlapping set of peptides, (ii) production of neutralization escape variants against each MAb and (iii) sequencing of neutralization escape variants. The study has shown that the virus has at least three overlapping linear neutralizing epitopes within a major antigenic site on VP1. The presence of a second, conformational site was demonstrated but its position on the virus particle was not located. Synthetic peptides with sequences representing the major site elicit antibodies which have similar broad cross-neutralizing activity to polyclonal serum or neutralizing MAbs produced with the virus against a range of field isolates.

Keywords: FMDV, epitope mapping, neutralization escape mutants

Received 20 June 1988; accepted 10 October 1988.

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