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J Gen Virol 70 (1989), 2563-2571; DOI 10.1099/0022-1317-70-10-2563
© 1989 Society for General Microbiology
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Identification of Virus-specific Polypeptides by Monoclonal Antibodies against Serotype 2 Marek's Disease Virus

Kazuhiro Nakajima1, Takeshi Shibayama1, Miya Yokota1, Kazuyoshi Ikuta1, Shiro Kato1 and Kanji Hirai2

1 Department of Pathology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565
and2 Department of Virology and Immunology, Medical Research Institute, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, Tokyo 113, Japan

A total of 41 antibody-secreting hybridoma cells against the HPRS24 strain of Marek's disease virus (MDV) type 2 (MDV2) have been isolated. Of these monoclonal antibodies (MAbs), 24 were found by immunofluorescence tests to react specifically with MDV2-infected cells, but not MDV type 1 (MDV1)- or herpesvirus of turkeys (HVT)-infected cells, while eight reacted with MDV1- or MDV2-infected cells and nine with MDV1-, MDV2- or HVT-infected cells. By using these MAbs, seven classes of MDV type-specific or cross-reactive polypeptides were characterized by immunoprecipitation followed by SDS-PAGE. Among them, a 28K/32K glycoprotein differed from the previously identified gA and gB. The 28K/32K glycoprotein was found on the surface of MDV2-infected cells and in the cytoplasm by an immunofluorescence test with MAbs. In addition, a cross-reactive polypeptide of 25K/29K was also detected in MDV1-infected cells with MAbs reactive with the 28K/32K glycoprotein of MDV2.

Keywords: MDV2, MAbs, polypeptides

Received 22 February 1989; accepted 15 June 1989.




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Copyright © 1989 by the Society for General Microbiology.