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Recombinant Vaccinia Virus Expressing the Herpes Simplex Virus Type 1 Glycoprotein C Protects Mice against Herpes Simplex Virus Challenge

Malcolm Bennett

Department of Microbiology
¹ Department of Biochemistry, Colleges of Liberal Arts and Veterinary Medicine, University of Tennessee, Knoxville, Tennessee 37996, U.S.A.

The gene encoding the herpes simplex virus type 1 (HSV-1) glycoprotein C (gC) was isolated and cloned into a vaccinia virus insertion vector, and the resulting vaccinia-gC vector was used to construct a recombinant vaccinia virus that expressed gC (VVgC5). Infection of cells with VVgC5 resulted in cell surface expression of authentic HSV-1 gC. HSV-1 gC-specific neutralizing antibodies were produced in VVgC5-immunized mice, and lymphocytes exhibited an HSV-1-specific proliferation response in vitro following infection. More importantly, VVgC5-immunized mice were resistant to subsequent lethal HSV-1 challenge.

Keywords: HSV-1, vaccinia virus, glycoprotein C

Present address: Department of Veterinary Pathology, University of Liverpool, Neston, Merseyside L64 7TE, U.K.

Received 10 March 1989; accepted 8 June 1989.