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Comparison of Antigenic Sites of Subtype-specific Respiratory Syncytial Virus Attachment Proteins

¹ University of Rochester, School of Medicine and Dentistry, Rochester, New York 14642
The² Rochester General Hospital, Department of Medicine, 1425 Portland Avenue, Rochester, New York 14621
and³ Praxis Biologics, 30 Corporate Woods, Rochester, New York 14623, U.S.A.

A panel of 19 monoclonal antibodies (MAbs) were used to probe the antigenic relationships between the G (attachment) proteins of A and B respiratory syncytial virus (RSV) subtypes (G_A and G_B). At least three and two antigenic sites were present on G_A and G_B, respectively, including a shared neutralizing site. Most of the antibodies had some degree of complement-independent neutralizing capacity, but in common was a large neutralization-resistant fraction of virus (range 13 to 78%). Passive administration of MAbs to the cross-reactive antigenic site reduced pulmonary virus titres of both A and B subtype virus in the cotton rat model. Protection with subtype-specific MAbs, however, did not always correlate with in vitro neutralizing capacity. The cross-reactive antigenic site appears to be stable to denaturation by polyacrylamide gel electrophoresis and is present on the unglycosylated and partially glycosylated forms of G_A and G_B by Western blot analysis of infected cell lysates.

Keywords: RSV, attachment protein, epitope mapping, animal protection

Received 14 February 1989; accepted 9 July 1989.

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