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Expression of the Human Parvovirus B19 Protein Fused to Protein A in Escherichia coli: Recognition By IgM and IgG Antibodies in Human Sera

¹ Service de Bacteriologie et Virologie
and² Laboratoire d'Hématologie Expérimentale, Centre Hayem, Hôpital Saint-Louis, 1 avenue Claude Vellefaux, Université Paris VII, 75010 Paris, France
and³ Institut für Hygiene und Medizinische Mikrobiologie, Universität Bern, CH 3010 Bern, Switzerland

A 1.4 kb fragment (nucleotides 2430 to 3901) encoding portions of the human parvovirus B19 structural proteins was inserted into the pRIT2 plasmid expression vector containing the gene encoding staphylococcal Protein A under the control of the phage promoter P_R. The fusion protein was used to raise antibodies in rabbits. The sera were shown by immune electron microscopy to agglutinate B19 particles and were also shown to recognize the VP2 B19 capsid protein, by Western blot analysis. The B19 antigenicity of the fusion protein was confirmed by immunoblot and enzyme immunoassay with IgG and IgM anti-B19-positive reference human sera.

Keywords: parvovirus B19, fusion protein, antigenic recognition

Received 23 January 1989; accepted 22 June 1989.

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