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Location of Neutralizing Epitopes on the Fusion Protein of Newcastle Disease Virus Strain Beaudette C

K. Yusoff^1,

¹ Department of Biochemistry and Genetics, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, U.K.
² National Institute for Veterinary Research, 1180 Brussels, Belgium
and³ Central Veterinary Laboratory, New Haw, Weybridge, Surrey KT15 3NB, U.K.

A panel of eight neutralizing monoclonal antibodies (MAbs) against the fusion (F) protein of Newcastle disease virus (NDV) has been shown to locate a major antigenic site on the basis of competitive binding assay and additivity index studies. Five epitopes (A1 to A5) have been located within this site on the F protein of the Beaudette C strain of NDV on the basis of cross-resistance plaque assays of MAb-resistant mutants raised against these MAbs. Epitopes A1, A4 and A5 are distinct; epitope A2 partially overlaps epitope A3. Nucleotide sequence analysis of the F genes of MAb-resistant mutants showed that each predicted single amino acid substitutions ranging from amino acid residues 157 to 171 for epitope A4 and at residues 72, 78, 79 and 343 for epitopes A1, A2, A3 and A5 respectively. These locations indicate that both the F1 and F2 fragments are involved in the formation of a single antigenic site and suggest the involvement of extensive protein folding in the active form of this F protein.

Keywords: NDV, fusion protein, epitope mapping, MAb

Present address: Department of Biochemistry and Microbiology, University of Pertanian, 43400 Serdang, Selangor, Malaysia.

Received 24 May 1989; accepted 7 July 1989.

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