| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Canto Blanco, 28049 Madrid, Spain
Antigenic variants of foot-and-mouth disease virus (FMDV) of serotype C (isolate C-S8c1) were selected upon serial passage of the virus in cell culture in the absence of anti-FMDV antibodies. The variants rose from frequencies of < 10-2 in the initial plaque-purified FMDV C-S8c1 preparation, to 0.1 to 1 in three passaged populations. The proportion of antigenic variants was quantified using a new in situ plaque immunotest. A nitrocellulose filter is applied to the agar overlay of a FMDV plaque assay, and allows recovery of infectious virus from individual plaques. A second filter is placed directly on the cell monolayer and binds enough virus to permit colorimetric visualization of plaques by an enzyme-linked assay using monoclonal antibodies (MAbs). Either all or a fraction of plaques from passaged FMDV failed to react with MAb 4G3, an antibody that recognizes an epitope located within residues 144 to 150 of capsid protein VP1. Some variants rapidly dominated the viral population, and others were maintained at low levels. RNA from unreactive viruses included mutations that resulted in amino acid substitutions at the epitope recognized by MAb 4G3. We discuss models for the selection of antigenic variants of FMDV in the absence of antibodies, and implications for the antigenic diversification of RNA viruses.
Keywords: FMDV, monoclonal antibody, mutation, RNA variability
Received 3 July 1989;
accepted 10 August 1989.
This article has been cited by other articles:
|
|
 |
|
  L. Aragones, A. Bosch, and R. M. Pinto Hepatitis A Virus Mutant Spectra under the Selective Pressure of Monoclonal Antibodies: Codon Usage Constraints Limit Capsid Variability J. Virol., February 15, 2008; 82(4): 1688 - 1700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Carrillo, Z. Lu, M. V. Borca, A. Vagnozzi, G. F. Kutish, and D. L. Rock Genetic and Phenotypic Variation of Foot-and-Mouth Disease Virus during Serial Passages in a Natural Host J. Virol., October 15, 2007; 81(20): 11341 - 11351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Cottam, D. T. Haydon, D. J. Paton, J. Gloster, J. W. Wilesmith, N. P. Ferris, G. H. Hutchings, and D. P. King Molecular Epidemiology of the Foot-and-Mouth Disease Virus Outbreak in the United Kingdom in 2001 J. Virol., November 15, 2006; 80(22): 11274 - 11282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Rieder, T. Henry, H. Duque, and B. Baxt Analysis of a Foot-and-Mouth Disease Virus Type A24 Isolate Containing an SGD Receptor Recognition Site In Vitro and Its Pathogenesis in Cattle J. Virol., October 15, 2005; 79(20): 12989 - 12998. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Grubman and B. Baxt Foot-and-Mouth Disease Clin. Microbiol. Rev., April 1, 2004; 17(2): 465 - 493. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Robles-Sikisaka, K. W. Hasson, D. K. Garcia, K. E. Brovont, K. D. Cleveland, K. R. Klimpel, and A. K. Dhar Genetic variation and immunohistochemical differences among geographic isolates of Taura syndrome virus of penaeid shrimp J. Gen. Virol., December 1, 2002; 83(12): 3123 - 3130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Arias, E. Lázaro, C. Escarmís, and E. Domingo Molecular intermediates of fitness gain of an RNA virus: characterization of a mutant spectrum by biological and molecular cloning J. Gen. Virol., May 1, 2001; 82(5): 1049 - 1060. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. T. Haydon, A. D. Bastos, N. J. Knowles, and A. R. Samuel Evidence for Positive Selection in Foot-and-Mouth Disease Virus Capsid Genes From Field Isolates Genetics, January 1, 2001; 157(1): 7 - 15. [Abstract] [Full Text]
|
|
|
|
|
|
C. M. Ruiz-Jarabo, N. Sevilla, M. Dávila, G. Gómez-Mariano, E. Baranowski, and E. Domingo Antigenic properties and population stability of a foot-and-mouth disease virus with an altered Arg-Gly-Asp receptor-recognition motif J. Gen. Virol., August 1, 1999; 80(8): 1899 - 1909. [Abstract] [Full Text]
|
|
|
|
|
|
E. Baranowski, N. Sevilla, N. Verdaguer, C. M. Ruiz-Jarabo, E. Beck, and E. Domingo Multiple Virulence Determinants of Foot-and-Mouth Disease Virus in Cell Culture J. Virol., August 1, 1998; 72(8): 6362 - 6372. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
