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Antibody-forming Cell Assays of Avian Paramyxoviruses: the Serotype-specific Response of Mice

Department of Veterinary Pathology, The Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, U.K.

Mice were immunized with eight different serotypes of avian paramyxovirus (PMV). The number of splenocytes that formed antibody to PMV-infected Madin-Darby bovine kidney cells was determined by immunoperoxidase staining. Microscopic examination revealed brown plaques of cell surface or inclusion body-like antigen comprising the glycoprotein or nucleoprotein-polymerase complex, respectively. The IgG response to virus glycoprotein was priming-dependent and 99% serotype-specific, a value which exceeds that of PMV typing using avian serology. The response to inclusion body-like antigens was more variable and less priming-dependent and suggested cross-reactivities between PMV-1 and PMV-3 or -9. The primary IgM response also included a 1 to 10% non-specific antibody response to host antigens revealed by neuraminidase treatment.

Keywords: paramyxoviruses, avian, serotype, AFC assay

Received 20 May 1988; accepted 7 October 1988.