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The Synthesis and Maturation of a Non-structural Extracellular Antigen from Tick-borne Encephalitis Virus and Its Relationship to the Intracellular NS1 Protein

Division of Biologics, PHLS, Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire SP4 0JG, U.K.

The replication of flaviviruses results in the secretion of four virus-coded proteins into the extracellular environment. Three of these proteins, E, C and M (or pre-M), are found in purified virions. A fourth virus-specified extracellular protein which was not present in either the slowly sedimenting haemagglutinin particles or in virions is described. The relationship of this protein to the intracellular NS1 polypeptide was investigated along with its similarity to the soluble complement-fixing antigen (SCF) reported for mosquito-borne flaviviruses. The difference in the M_r of NS1 and SCF is the result of additional glycosylation of SCF, mostly by the addition of fucose molecules. The synthesis of E and NS1 is sequential but their secretion is simultaneous, suggesting a role for NS1 in virion protein transport or virion release.

Keywords: tick-borne encephalitis, membrane proteins, NS1

Received 13 May 1988; accepted 13 October 1988.

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