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Properties of Three Spontaneous Mutants of Red Clover Necrotic Mosaic Virus

Department of Pure and Applied Biology, Imperial College of Science, Technology and Medicine, Prince Consort Road, London SW7 2BB, U.K.

Three spontaneous mutants of strain TpM-34 of red clover necrotic mosaic virus, designated M-A, M-B and M-C, were isolated. In gel diffusion tests the three mutants were serologically indistinguishable from the parent but when inoculated onto Vigna unguiculata each induced characteristic symptoms which differed from those induced by TpM-34. TpM-34 induced similar numbers of lesions at 17 °C and 26 °C. However, although inoculation with RNA of the mutants induced 70 to 80% as many lesions as inoculation with TpM-34 RNA at 17 °C, very few lesions were induced by any of the mutants at 26 °C. The symptoms induced by heterologous mixtures of RNA 1 or RNA 2 of TpM-34 with RNA 2 or RNA 1 of each mutant indicated that M-A, M-B and M-C each has mutations in both RNA components, but that symptom expression was determined predominantly by RNA 1.

Keywords: RCNMV, pseudorecombinants, symptom expression

Received 9 August 1988; accepted 26 October 1988.