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Immunoblotting Reveals Differences in the Accumulation of Envelope Protein by Wild-type and Vaccine Strains of Yellow Fever Virus

P. A. Cane

NERC Institute of Virology, Mansfield Road, Oxford OX1 3SR, U.K.

Seventeen strains of yellow fever virus were compared by immunoblotting using polyclonal and monoclonal antibodies. Strains originating in South America were readily distinguishable from African strains on the basis of differences in the envelope protein. The 17D vaccine strain and strains derived from it differed radically from the parent Asibi and other wild-type strains in that cells infected with 17D did not accumulate intact envelope protein. We suggest that failure to accumulate the major viral surface protein is due to its increased susceptibility to breakdown and that this property may be a contributing factor in the attenuation of the vaccine strains.

Keywords: yellow fever virus, envelope protein, strain variation

Present address: Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, U.K.

Received 14 July 1988; accepted 3 November 1988.