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J Gen Virol 70 (1989), 659-672; DOI 10.1099/0022-1317-70-3-659
© 1989 Society for General Microbiology
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Induction of Transmissible Gastroenteritis Coronavirus-neutralizing Antibodies in vitro by Virus-specific T Helper Cell Hybridomas

M. J. Bullido, I. Correa, G. Jiménez, C. Suñé, F. Gebauer and L. Enjuanes

Centro de Biología Molecular, CSIC-Universidad Autónoma, Canto Blanco, 28049 Madrid, Spain

Three transmissible gastroenteritis (TGE) virus-specific T helper (Th) cell hybridomas have been generated from virus-primed BALB/c mice, by fusion with the thymoma BW5147. The hybridomas responded to purified u.v.-inactivated TGE virus with interleukin production and growth inhibition. TGE virus recognition by the hybridomas was restricted by the major histocompatibility complex: only splenocytes from syngeneic or semi-syngeneic mice were able to recognize the antigen. The three hybridomas were Thy 1.2+, but did not express detectable levels of Lyt 1 or Lyt 2 antigens by fluorescent cell sorting analysis. Only one hybridoma (T. 1J. B5) expressed the L3T4 marker. These hybridomas had helper activity, as they were able to reconstitute in vitro the synthesis of TGE virus-specific antibodies by Th cell-depleted spleen cells from immune BALB/c mice. The antibodies that they induced specifically neutralized by 103- to 104-fold the infectivity of TGE virus, ruling out the possibility of inhibition of virus replication by interferon. These hybridomas could be very useful for identifying antigenic domains in TGE virus recognized by Th cells, which cooperate with B cells in the synthesis of neutralizing antibodies.

Keywords: TGE virus, Th cell hybridomas, neutralizing antibodies

Received 14 June 1988; accepted 25 October 1988.




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Copyright © 1989 by the Society for General Microbiology.