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1 Department of Veterinary Microbiology, University of Saskatchewan
2 Biostar, Inc., P.O. Box 1000, Sub P.O. 6
and3 Veterinary Infectious Disease Organization, 124 Veterinary Road, Saskatoon, Saskatchewan, Canada, S7N 0W0
Six monoclonal antibodies (MAbs) to bovine coronavirus (BCV, Quebec isolate) E2 and E3 glycoproteins which were found previously to be neutralizing in vitro were examined for virus-neutralizing activity in vivo. Surgically ligated intestinal loops of newborn colostrum-deprived calves were virus-inoculated, mock-infected or inoculated with a mixture of virus and antibody. Of the six BCV-specific MAbs, four were found to be protective against a virulent field isolate of BCV, as indicated by a reduction in villous atrophy. These MAbs were specific to antigenic domain A and antigenic domains A1 and A2 on the E2 and E3 glycoproteins respectively. MAbs to antigenic domains B and C on the E2 and E3 glycoproteins, respectively, were not protective.
Keywords: bovine coronavirus, monoclonal antibodies, E1, E2 glycoproteins
Present address: Animal Diseases Research Institute, P.O. Box 640, Lethbridge, Alberta, Canada, T1J 3Z4.
Received 7 July 1988;
accepted 18 November 1988.
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