HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Stokes, A. Articles by Murray, P. K. Search for Related Content PubMed PubMed Citation Articles by Stokes, A. Articles by Murray, P. K. Agricola Articles by Stokes, A. Articles by Murray, P. K.

A Hamster Model of Equine Herpesvirus Type 1 (EHV-1) Infection; Passive Protection by Monoclonal Antibodies to EHV-1 Glycoproteins 13, 14 and 17/18

¹ Department of Immunology, AFRC Institute for Animal Health, Pirbright Laboratory, Guildford, Surrey GU24 0NF, U.K.
and² Department of Veterinary Science, University of Kentucky, Lexington, Kentucky 40546-0076, U.S.A.

Following intraperitoneal or intranasal inoculation of the Syrian hamster with equine herpesvirus type 1 (EHV-1), strain Kentucky D, virus replicated in the liver and lungs reaching a peak at 4 days post-infection (p.i.). By day 6 p.i. virus titres in these organs had reduced and the spleen contained virus-specific cytotoxic cells. This cytotoxicity was mediated by T cells since treatment of effector cells with a monoclonal antibody to hamster T lymphocytes inhibited the effect. An antiviral humoral immune response was present by day 4 when antibodies capable of lysing EHV-1-infected target cells in the presence of complement were detected. The transfer of serum from recovered hamsters into naive recipients 24 h before challenge prevented virus infection, whereas serum transferred 24 h after challenge reduced the titre of virus recovered from target organs. Inoculation of hamsters with monoclonal antibodies directed to glycoproteins 13, 14 and 17/18 of a subtype 1 virus (Army 183) before virus challenge protected hamsters. This hamster model will prove useful for studying the immune response to EHV-1 and evaluating the immunogenicity of individual virus components.

Keywords: EHV-1, hamster model, glycoproteins

Received 17 October 1988; accepted 26 January 1989.