HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Stewart, J. P. Articles by Arrand, J. R. Search for Related Content PubMed PubMed Citation Articles by Stewart, J. P. Articles by Arrand, J. R. Agricola Articles by Stewart, J. P. Articles by Arrand, J. R.

Enhanced Expression of the Epstein-Barr Virus Latent Membrane Protein by a Recombinant Vaccinia Virus

Hans-Werner Heinrich

Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Wilmslow Road, Manchester M20 9BX, U.K.

The complete coding sequence of the Epstein-Barr virus strain B95-8 latent membrane protein (LMP) was cloned using a Raji cell cDNA library and genomic B95-8 DNA. The clone was characterized by sequencing and then used to make a recombinant vaccinia virus. This virus (VLMP) was shown to express a relatively high level of LMP in an authentic fashion. Antisera raised in rabbits against VLMP were shown to react with B95-8 LMP as well as cross-reacting with a 50K cellular protein.

Keywords: EBV, latent membrane protein, vaccinia virus

Present address: VEB Friedrich-Loeffler Institut, 2201 Insel Riems, G.D.R.

Received 7 November 1988; accepted 11 January 1989.

This article has been cited by other articles:

				M. N. Wakeling, D. J. Roy, A. A. Nash, and J. P. Stewart Characterization of the murine gammaherpesvirus 68 ORF74 product: a novel oncogenic G protein-coupled receptor J. Gen. Virol., May 1, 2001; 82(5): 1187 - 1197. [Abstract] [Full Text]