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Use of Monoclonal Antibodies in the Purification of an Inhibitor of Virus Replication by Affinity Chromatography

¹ Virus Laboratory, Agricultural Research Organization, The Volcani Center, Bet Dagan
and² George S. Wise Faculty of Life Sciences, Tel-Aviv University, Ramat-Aviv, Israel

Mouse monoclonal antibodies (MAbs) were prepared to an inhibitor of virus replication (IVR), released from protoplasts or leaf tissue of hypersensitive tobacco plants infected with tobacco mosaic virus. The MAbs were highly specific for IVR and reduced its antiviral activity. Using these MAbs in affinity chromatography enabled the recovery of purified IVR. SDS-PAGE of the immunoaffinity-purified IVR gave a single M_r 23K band. Immunoblots of IVR from extracts of protoplast or leaf tissue also revealed a single M_r 23K band which suggests that protoplast and tissue IVR are closely related.

Keywords: IVR, hypersensitivity, monoclonal antibodies

Received 9 August 1988; accepted 11 January 1989.