J Gen Virol Try IJSEM Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 70 (1989), 1321-1327; DOI 10.1099/0022-1317-70-6-1321
© 1989 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Storey, C. C.
Right arrow Articles by Bacon, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Storey, C. C.
Right arrow Articles by Bacon, J.
Agricola
Right arrow Articles by Storey, C. C.
Right arrow Articles by Bacon, J.

Further Characterization of a Bacteriophage Recovered from an Avian Strain of Chlamydia psittaci

Christopher C. Storey, Meryl Lusher, Shirley J. Richmond and Jane Bacon{dagger}

Department of Medical Microbiology, Medical School, University of Manchester, Oxford Road, Manchester M13 9PL, U.K.

The genome of a 22 nm icosahedral phage which infects some avian Chlamydia psittaci strains recovered from domestic ducks has been characterized as a ss circular DNA molecule of about 4850 bases. The replicative form of this genome was isolated from purified chlamydial organisms. A restriction endonuclease cleavage site map of the genome was constructed from dsDNA synthesized in vitro from ss phage DNA and EcoRI fragments were then cloned into pUC9. The phage genome was detected only by Southern blot hybridization in C. psittaci which was productively infected with phage; no evidence was found for the integration of phage DNA into the chlamydial chromosome. Three viral polypeptides, of approximate Mr values 75K, 30K and 16.5K were identified when phage was analysed by SDS-PAGE. This virus, which we have designated Chp1, is either an aberrant member of the Microviridae or the first member of a new bacteriophage family.

Keywords: phage, Chlamydia psittaci, DNA, circular single strand

{dagger} Present address: Department of Veterinary Pathology, Royal School of Veterinary Studies, Edinburgh EH9 1QH, U.K.

Received 14 December 1988; accepted 14 February 1989.


This article has been cited by other articles:


Home page
 Mol Biol EvolHome page
L. Gavotte, H. Henri, R. Stouthamer, D. Charif, S. Charlat, M. Bouletreau, and F. Vavre
A Survey of the Bacteriophage WO in the Endosymbiotic Bacteria Wolbachia
Mol. Biol. Evol., February 1, 2007; 24(2): 427 - 435.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. D. Read, G. S. A. Myers, R. C. Brunham, W. C. Nelson, I. T. Paulsen, J. Heidelberg, E. Holtzapple, H. Khouri, N. B. Federova, H. A. Carty, et al.
Genome sequence of Chlamydophila caviae (Chlamydia psittaci GPIC): examining the role of niche-specific genes in the evolution of the Chlamydiaceae
Nucleic Acids Res., April 15, 2003; 31(8): 2134 - 2147.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
R.-c. Hsia, L.-M. Ting, and P. M. Bavoil
Microvirus of Chlamydia psittaci strain Guinea pig Inclusion Conjunctivitis: isolation and molecular characterization
Microbiology, July 1, 2000; 146(7): 1651 - 1660.
[Abstract] [Full Text]

Home page
 J. Virol.Home page
B. L. Liu, J. S. Everson, B. Fane, P. Giannikopoulou, E. Vretou, P. R. Lambden, and I. N. Clarke
Molecular Characterization of a Bacteriophage (Chp2) from Chlamydia psittaci
J. Virol., April 15, 2000; 74(8): 3464 - 3469.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1989 by the Society for General Microbiology.