HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Rota, P. A. Articles by Kendal, A. P. Search for Related Content PubMed PubMed Citation Articles by Rota, P. A. Articles by Kendal, A. P. Agricola Articles by Rota, P. A. Articles by Kendal, A. P.

Cross-protection against Microvariants of Influenza Virus Type B by Vaccinia Viruses Expressing Haemagglutinins from Egg- or MDCK Cell-derived Subpopulations of Influenza Virus Type B/England/222/82

Division of Viral Diseases, Center for Infectious Diseases, Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30333, U.S.A.

B/Singapore/222/79-like influenza viruses isolated from three patients during the winter of 1981 to 1982 and cultured in either embryonated hens' eggs or MDCK cells were studied. Sequence analysis indicated that the haemagglutinin (HA) genes of the six virus preparations contained at least four distinct HA1 sequences which differed by up to six amino acids. Only one pair of viruses had amino acid differences between the egg- and MDCK cell-derived viral subpopulations and this change did not affect a glycosylation site. Mice infected with previously described recombinant vaccinia viruses expressing either the egg- or MDCK cell-derived HA of B/England/222/82 developed neutralizing antibodies against all of the 1982 type B viruses and were protected against intranasal challenge with these viruses. Therefore, in this model system, the minor sequence variation between the HAs of egg- and MDCK cell-derived influenza B/England/222/82 virus had no detectable effect on the induction of cross-protection.

Keywords: influenza B virus, haemagglutinins, cross-protection

Received 7 November 1988; accepted 20 February 1989.

This article has been cited by other articles:

				I. V. Alymova, S. Kodihalli, E. A. Govorkova, B. Fanget, C. Gerdil, and R. G. Webster Immunogenicity and Protective Efficacy in Mice of Influenza B Virus Vaccines Grown in Mammalian Cells or Embryonated Chicken Eggs J. Virol., May 1, 1998; 72(5): 4472 - 4477. [Abstract] [Full Text] [PDF]