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Molecular Analysis of Agaricus bisporus Double-stranded RNA

¹ Department of Plant Biology, University of Groningen, Kerklaan 30, 9751 NN Haren
and² Mushroom Experimental Station, Horst, The Netherlands

A detailed analysis was made of dsRNA molecules present in Agaricus bisporus (cultivated mushroom) affected and non-affected by La France disease occurring in The Netherlands. A specific pattern of 10 major dsRNA molecules was always associated with affected strains but non-affected strains also contained at least one of the dsRNAs. Cross-hybridization under stringent conditions showed that the dsRNAs were all unique.

Keywords: Agaricus bisporus, La France disease, fungal virus

Received 22 August 1988; accepted 8 February 1989.