HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Dubuisson, J. Articles by Pastoret, P.-P. Search for Related Content PubMed PubMed Citation Articles by Dubuisson, J. Articles by Pastoret, P.-P. Agricola Articles by Dubuisson, J. Articles by Pastoret, P.-P.

Proteins Specified by Bovine Herpesvirus Type 4: Structural Proteins of the Virion and Identification of Two Major Glycoproteins by Using Monoclonal Antibodies

Department of Virology-Immunology, Faculty of Veterinary Medicine, University of Liège, Institut de Chimie-B6 (R80), 4000 Liège, Belgium

Bovine herpesvirus type 4 proteins were identified by PAGE of [³⁵S]methionine- or [³H]glucosamine-labelled purified virions. Thirty-one monoclonal antibodies (MAbs) raised against the V. Test strain were used to identify 29 proteins, ten of which were glycosylated. All of these glycoproteins belonged to the viral envelope and a 140K nonglycosylated protein appeared to be the major nucleocapsid protein. The MAbs were classified into two groups. The first group precipitated three glycoproteins of M_r 150K, 120K and 51K. The 120K and 51K glycoproteins were linked by disulphide bonds and the 150K glycoprotein was linked to the others by non-covalent bonds. The second group precipitated a different 120K glycoprotein.

Keywords: herpesvirus, bovine, monoclonal antibodies, structural proteins

Received 23 December 1988; accepted 21 March 1989.

This article has been cited by other articles:

				V. Keuser, F. Schynts, B. Detry, A. Collard, B. Robert, A. Vanderplasschen, P.-P. Pastoret, and E. Thiry Improved Antigenic Methods for Differential Diagnosis of Bovine, Caprine, and Cervine Alphaherpesviruses Related to Bovine Herpesvirus 1 J. Clin. Microbiol., March 1, 2004; 42(3): 1228 - 1235. [Abstract] [Full Text] [PDF]

				L. Gillet, F. Minner, B. Detry, F. Farnir, L. Willems, M. Lambot, E. Thiry, P.-P. Pastoret, F. Schynts, and A. Vanderplasschen Investigation of the Susceptibility of Human Cell Lines to Bovine Herpesvirus 4 Infection: Demonstration that Human Cells Can Support a Nonpermissive Persistent Infection Which Protects Them against Tumor Necrosis Factor Alpha-Induced Apoptosis J. Virol., March 1, 2004; 78(5): 2336 - 2347. [Abstract] [Full Text] [PDF]

				G. Donofrio, S. Cavirani, and V. L. van Santen Establishment of a cell line persistently infected with bovine herpesvirus-4 by use of a recombinant virus J. Gen. Virol., July 1, 2000; 81(7): 1807 - 1814. [Abstract] [Full Text]